Intracellular adhesion gene expression studies in biofilm-forming of Staphylococcus aureus isolated from different clinical sources in Bagdad. Iraq.

Articles in Press

Document Type : Original Article

Authors

Al-Bayan University, College of Dentistry. Baghdad, Iraq

Abstract

Background: Staphylococcus aureus (S. aureus) a pathogenic bacterium typically found in the population is noteworthy for its pathogenicity due to its ability to produce biofilms with high virulence. Aِim: To identify the gene icaA, which is responsible for biofilm formation in clinical isolates from wounds and urine samples. Methods: This gene is involved in the construction of slime layers. The present study collected a total of 425 clinical samples, including urine and wound swabs. We used four different methods to identify S. aureus isolates: the biochemical test methodology, the Analytical Profile Index (API) Staph system, the Vitek-2 compact systems, and the polymerase chain reaction (PCR) targeting the 16SrRNA. In the end, we confirmed only 388 isolates to be S. aureus. Results: We assessed the ability of S. aureus isolates to produce biofilm using 96-well microtiter plates. The examination of 388 isolates revealed that 146 isolates (37.6%) formed robust biofilms, 160 isolates (41.3%) formed moderate biofilms, and 82 isolates (21.1%) formed weak biofilms. Additionally, the expression level of the strong biofilm-producing isolate (6.508) was considerably lower (P<0.01) compared to the weak and moderate isolates. The results indicated that the moderate isolate's expression level was higher than that of the weak isolate. Conclusion: A quantitative PCR analysis was conducted on three isolates with different biofilm forming capacity (EPS) to evaluate the transcript levels of the icaA gene, which is responsible for exopolysaccharide production.

Keywords

Main Subjects

Microbes and Infectious Diseases

Articles in Press, Accepted Manuscript
Available Online from 17 November 2024

Files

Share

How to cite

Statistics