Detection of ESBL resistance Escherichia coli from vesico vaginal fistula (VVF) patients attending VVF Hospital Birnin Kebbi

Aliero, Adamu Almustapha; Bagudo, Ahmad Ibrahim; Muhammad, Amina; Abdullahi Aliero, Saadatu; Manga, Sule Sahabi; Ntulume, Ibrahim; Dorcas, Joshua Ushe

doi:10.21608/mid.2024.317032.2186

Detection of ESBL resistance Escherichia coli from vesico vaginal fistula (VVF) patients attending VVF Hospital Birnin Kebbi

Articles in Press

Document Type : Original Article

Authors

¹ Department of Microbiology, Faculty of Life Sciences, Kebbi State University of Science and Technology, Aliero, Nigeria

² Department of Microbiology, Faculty of Life Sciences, Kebbi State Univeristy of Science and Technology, Aliero, Nigeria

³ Department of Microbiology, Faculty of Life Sciences. Kebbi State University of Science and Technology, Aliero, Nigeria

⁴ Department of Pharmacology and Therapeutics, School of Medicine, Health and Life Sciences, King Ceasor University, Kampala Uganda

10.21608/mid.2024.317032.2186

Abstract

Background: Uropathogenic Escherichia coli (UPEC) associated with Vesico Vaginal Fistula (VVF) is now a global problem, especially with the current increase in antibiotic resistance among the UPEC. This research was designed to detect ESBLS-resistant E. coli from Vesico Vaginal Fistula (VVF) patients attending VVF Hospital Birnin Kebbi. Methods: A hospital-based cross-sectional study was conducted at Vesico Vaginal Fistula Center Birnin Kebbi. A total of 280 urine samples were collected from the consented patients and analysed using standard microbiological methods to detect ESBLS-resistant E. coli. The isolated E. coli was identified using morphological and biochemical tests. ESBLS resistance was confirmed using the double-disc synergy method with ceftazidime (30 μg) and amoxicillin-clavulanic acid (20 μg/10 μg). Results: Of the 280 urine samples collected, 167 (59.62%) were positive with significant bacteriuria growth. Eighty-five (85) uropathogenic E. coli were identified. The results of the antibiotic-resistant pattern of the E. coli isolated from the VVF patients showed that 57 (67.06%) of the isolates were resistant to chloramphenicol, and a low-resistant 14 (16.47%) were observed from Nitrofurantoin. The results of the phenotypic confirmatory disc diffusion test showed that 34 (80.00%) E. coli isolates were positive for ESBL, while the double disc synergy test shows that 29 (72.50%) were ESBL producers. Conclusion: The findings revealed that some VVF patients harboured uropathogenic E. coli that are ESBL producers.

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