Comparison of agreement between the BioFire Film Array Pneumonia Panel and conventional culture-based methods in the diagnosis of bacterial pneumonia.

Articles in Press

Document Type : Original Article

Authors

1 Department of Clinical Bacteriology, Mohammed V Military Teaching Hospital, Faculty of Medicine and Pharmacy of Rabat, Mohammed V University, Rabat, Morocco

2 Laboratory of Microbiology, Mohammed VI University Hospital, Faculty of Medicine and Pharmacy (University Mohammed the First), Oujda, Morocco

Abstract

Background: Acute bacterial pneumonia is a bacterial infection of the lower respiratory tract caused by a bacterial agent. Diagnosis is delicate and depends on clinical, radiological and biological data. The aim of this study was to compare the agreement of the BioFire ® FilmArray ® Pneumonia Panel test with conventional methods used in the microbiological diagnosis of bacterial pneumonia. Methods: This was a retrospective study carried out in the bacteriology department. Samples were collected by conventional bacteriological methods and molecular methods using Biofire® FilmArray © Pneumonia panel. Results: The PN correctly detected all bacterial targets isolated by culture if the bacteria were included in the panel targets, with a positive agreement of 100%. Negative agreement ranged from 60 to 93%, and overall negative agreement was 79.6%.  Overall positive and negative concordance for the detection of strains harboring CTX-M, NDM and MecA/MecC/MREJ genes were 100.0% and 94% respectively. Conclusion: PN showed good agreement with culture methods nevertheless an interpretation correlated with culture data and clinico-radiological data would be judicious for a better management of these pathologies.

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Main Subjects

Microbes and Infectious Diseases

Articles in Press, Accepted Manuscript
Available Online from 14 September 2024

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