Acinetobacter species Isolated from Different Cattle Samples in Ibadan, Nigeria is a Reservoir of Antibiotic Resistance Genes

Falodun, Olutayo I.; Zakari, Chubiyojo V.; Azu, Victor

doi:10.21608/mid.2024.310664.2141

Acinetobacter species Isolated from Different Cattle Samples in Ibadan, Nigeria is a Reservoir of Antibiotic Resistance Genes

Articles in Press

Document Type : Original Article

Authors

¹ Microbiology Department, University of Ibadan, Ibadan Nigeria

² Department of Microbiology, University of Ibadan, Ibadan, Nigeria

³ Bioreprository Clinical Virology University College, Ibadan, Nigeria

10.21608/mid.2024.310664.2141

Abstract

Background: Acinetobacter baumannii is an important pathogen of public health significance. The study was designed to determine the occurrence, antibiotic resistance pattern and presence of resistance genes in Acinetobacter spp. from different cattle waste samples in a major abattoir in Ibadan, Nigeria. Methods: Cattle waste samples including eviscerated, burnt effluent and dung were collected. Acinetobacter species were isolated using CHROMagar Acinetobacter medium (Paris, France) and identified using standard microbiological, biochemical and molecular techniques. Susceptibility test against antibiotics was by Kirby-Bauer disk diffusion technique on Mueller Hinton agar. Antibiotic resistance genes were detected using PCR. Results: Out of the 120 isolates obtained, 80(66.7%) were identified as Acinetobacter spp. comprising 70(58.3%) A. baumannii and 10(8.3%) A. calcoaceticus. Resistance of A. baumannii to tetracycline and ceftriaxone were 69(98.6%) and 64(91.4%), respectively, while that for A. calcoaceticus were 100% and 90.0%, respectively. Also, 17(21.3%) isolates were multi-drug resistant, while, the range of multiple antibiotic resistance index was between 0.3 and 0.9. This is an indication that the isolates were recovered from high-risk sources of contamination. Tetracycline and beta-lactam resistance genes detected in the Acinetobacter spp. were tet_M (58.8%), tet_A (53%)_,tet_L (11.3%)_,tet_B(5.9%) and bla_TEM (41.2%). Conclusion: The cattle waste samples haboured antibiotic resistant Acinetobacter spp. and their genes which could pose threat to public health. Therefore, measures that will prevent the spread of drug resistant bacteria in abattoirs should be adhered to.

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