Linking 5’-promoter region of Cdx2 VDR polymorphism and serum vitamin D association in female genital tuberculosis.

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Document Type : Original Article

Authors

1 Department of Obstetrics and Gynecology, King George’s Medical University, Lucknow (226003), Uttar Pradesh, India

2 Department of Obstetrics and Gynecology, All India Institute of Medical Sciences, Raebareli (229405), Uttar Pradesh, India

3 Department of Microbiology, King George’s Medical University, Lucknow (226003), Uttar Pradesh, India

Abstract

Background: Globally, genital tuberculosis (GTB) is a substantial cause of female infertility. The vitamin D receptor (VDR), a member of the nuclear receptor superfamily, mediates the immunological function of vitamin D3, activates macrophages, and vitamin D deficiency has been linked to female genital tuberculosis (FGTB) risk. Objective: The aim of this study was to assess the relationship between risk of FGTB and the vitamin D receptor gene polymorphism (Cdx2) and serum 25 (OH) D3 level.Methods: 150 confirmed FGTB cases and 150 healthy controls were recruited. Serum 25 (OH) D3 level was measured by ELISA. Genomic DNA was extracted and the genotyping of VDR-Cdx2 polymorphism was performed by Tetra-primer amplification refractory mutation system (T-ARMS-PCR).Results: Serum 25(OH) D3levels were significantly lower among FGTB patients. The frequency of A allele was 68% in FGTB and 48.6% in control; A allele was significantly associated with increased risk of FGTB [OR = 2.24; 95 % confidence interval CI=1.26-1.81; p< 0.0001].However, the frequency of G allele was 32% in FGTB and 51.3% in control; G allele did not show significant risk of FGTB [OR = 1.10; 95 % confidence interval CI=0.78-1.44; p = 0.67]. A significant association was found between VDR Cdx-2 AA (p<0.001) genotype and Serum 25(OH) D3level.Conclusion: Genotype frequencies of VDR gene polymorphism and serum vitamin D level were found to have significant association leads. VDR dysfunction could increase FGTB risk. Understanding the synergism between VDR polymorphism and serum vitamin D in FGTB will be important to identifying the new prognostic tool and target for therapy in serum vitamin D deficient individuals

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Microbes and Infectious Diseases

Articles in Press, Accepted Manuscript
Available Online from 17 January 2024

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